Recently, the Research Group of Supramolecular Photochemistry at the Institute of Physical and Chemical Technology, Chinese Academy of Sciences, developed for the first time a class of reactive fluorescent sensors that selectively detect glutathione (GSH) in living cells. Related research results were published in the Journal of the American Chemical Society recently. Free radical damage is one of the important molecular mechanisms of tissue damage. Many diseases, such as heart disease, Alzheimer's disease, Parkinson's disease and tumors, have free radicals involved in the damage mechanism. "Small biomolecules containing sulfhydryl groups, such as cysteine ​​(Cys), homocysteine ​​(Hcy), and GSH, will maintain the redox balance by removing excess free radicals in biological systems." Researcher Chen Yuzhe said. According to reports, as the most small thiol-containing biomolecule in the cell, GSH not only participates in the cellular antioxidant response and maintains the body's redox balance, but also participates in regulating cell proliferation, the body's immune response, and acting as a neuromodulator in the nervous system. And the role of neurotransmitters. However, the similarity of the structure and reactivity of small biomolecules containing sulfhydryl groups often makes fluorescent probes that generally detect GSH produce the same or similar responses to Cys and Hcy. Therefore, there is still a huge challenge to develop a fluorescent sensor that detects GSH with high selectivity. In the article, the research team reported a class of ratio-based fluorescent chemical sensors based on monochlorinated BODIPY derivatives. Unlike the traditional fluorescence detection mechanism, the research team used a brand new "two-step reaction" to distinguish GSH from Cys and Hcy. "Routine detection is mainly achieved by the reaction between the sulfhydryl group and the sensor, so it will produce a similar response to GSH, Cys and Hcy; and we use a novel two-step reaction mechanism, Cys and Hcy through the synergy of sulfhydryl and amino groups The reaction eventually produces an amino-substituted product, and GSH produces a thiol-substituted product, which causes a significant change in the spectrum, distinguishing it from Cys and Hcy. "Chen Yuzhe explained. Industry experts believe that the results will provide new methods for studying the effects of cancer, heart disease, aging and other diseases and the diagnosis and treatment methods. It is understood that the relevant research work has been supported by the National Natural Science Foundation of China Excellent Youth Science Foundation, the Ministry of Science and Technology "973" program and the Chinese Academy of Sciences "100 People Program" Pet Teeth Cleaning Finger Wipes Pet Teeth Cleaning Finger Wipes,Tooth Cleaning Wipe Dog,Tooth Cleaning Wipe Cats,Dogs Cats Tooth Cleaning ShangHai Youhang Technology Co.,LTD , https://www.yhecoclean.com