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Human mucosa-associated epithelial chemokine (MEC/CCL28) ELISA kit
[Human mucosa-associated epithelial chemokine (MEC/CCL28) ELISA kit] All products sold by Guangrui Bio are 100% factory-supplied, quality assurance, price concessions! Different Elisa kit brands, different Elisa kit prices, more species, good product quality, high sensitivity, free technical testing! Welcome to call the ELISA manufacturer to order: how to save the elisa kit: 1, the kit is stored: 2-8 ° C. 2. Validity: 6 months 1. Uniform adsorption, good adsorption, low blank value, high transparency at the bottom of the hole, high repeatability, high reliability. 3. Purchase ELISA kit, free test 4. Elisa kit technical service Requirements: professional, standardized, high efficiency 5, human, mouse, rat, guinea pig, rabbit, dog, chicken, horse, monkey, sheep, pig, cow, [human mucosa-associated epithelial chemokine (MEC / CCL28) ELISA reagent Box] crocin I5,6-xyleneimidazole 582-60-5DL-serine 302-84-1 peak α-amylase 9001-19-8 γ-aminobutyric acid 20791 iron tartrate 2944-68-5 quercetin Cinnamon oil 8015-91-6; 8007-80-5 adamantane, D-leucine 5534-09-2 aldehyde reagent 126-81-8(+)-catechin [human mucosa-associated epithelial chemokine (MEC) /CCL28) ELISA kit] Test sample processing method: 1. Urine: Collect with sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. The chest and ascites and cerebrospinal fluid are implemented with reference to this. 2. Tissue specimen: After cutting the specimen, weigh the weight. Add a certain amount of PBS, pH 7.4. It was quickly frozen and stored in liquid nitrogen for use. The specimen still maintains a temperature of 2-8 ° C after melting. Add a certain amount of PBS (pH 7.4), or tissue protein extraction reagent, and homogenize the specimen by hand or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. One part of the package is to be tested, and the rest is frozen for use. 3. Cell culture supernatant: When detecting secreted components, collect them in a sterile tube. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. 4. Plasma: EDTA, sodium citrate or heparin should be selected as anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. 5. Serum: The blood is naturally coagulated at room temperature for 10-20 minutes, and then centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again. 6. Culture the cells: When the components in the cells are detected, the cell suspension is diluted with PBS (pH 7.2-7.4), and the cell concentration reaches about 1 million/ml. By repeatedly freezing and thawing or adding tissue protein extraction reagents, the cells are destroyed and the intracellular components are released. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.