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Research Reagents-Japanese researchers explore the mechanism by which transposons in embryonic stem cells are suppressed
Japanese researchers explore the mechanism by which transposons are suppressed in embryonic stem cells The detailed mechanism of inhibition. This result is expected to be widely used in the research of induced multifunctional stem cells (iPS cells) and gene carriers. Transposons are fragments of genes with specific functions that can replicate themselves and move around in gene sequences. Some transposons can regulate gene expression and can induce gene mutations. Therefore, there is a mechanism for inhibiting transposons in vivo. Usually, this inhibition is achieved by DNA methylation. In embryonic stem cells, it has been found that there is an inhibition mechanism other than DNA methylation, but the details have not been explained. Researchers at the Institute of Virology, Kyoto University, Japan, and other researchers used embryonic stem cells from experimental mice to find that if an enzyme "ESET" is blocked, an endogenous retrovirus in The cells will become very active. Compared with when "ESET" works normally, the number of viruses has increased by 25 times. This shows that "ESET" is a kind of "switch" that controls the activity of the transposon. In the research of iPS cells and gene carriers, it is a very important technology to control the function of specific genes. The researchers said that this achievement is expected to be widely used in the research of iPS cells and gene carriers.