At the same time, the current and ion current of wheat protoplasts were measured, and it was found that the current is closely related to K + and has nothing to do with Ca2 +

Potassium is an essential element for plant growth and development, and the flow of ions determines the efficiency of potassium nutrition recycling. At present, many molecules responsible for ion transport have been isolated and identified, but the regulatory mechanism of the transport direction is currently unclear.

Researchers such as Matthew Gilliham of Cambridge University published a research paper in "The Plant Journal", using patch clamp technology to obtain the whole cell shape and current-voltage relationship of wheat root protoplasts, and detected the quality by "non-damage micro-measurement technology" The net ion flow rate of membrane K + and Ca2 + was compared with the current intensity measured by patch clamp technique. It was found that the change of K + flow rate / intensity ratio reflects the different distribution of KORC channels on the plasma membrane, and the activation of Ca2 + flow rate and K + channel There is no correlation.

Studies have shown that when a strong Ca2 + flow is detected, no current is generated. That is, when a patch clamp technique is used to study the Ca2 + channel, even if no current is detected, it is likely that there is a Ca2 + flow. Therefore, the number and type of ion carriers and ion channels can be accurately determined only by detecting the ion current and current across the membrane.