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Beijing human metalloproteinase tissue inhibitory factor-1 (TIMP-1) elisa test kit instructions
Human metalloproteinase tissue inhibitory factor-1 (TIMP-1) enzyme-linked immunoassay (ELISA) kit instructions for use This reagent is for research use only: This kit is used to determine the metalloproteinase tissue in human serum, plasma and related liquid samples Inhibitor-1 (TIMP-1) content. Experimental principle: This kit uses the double antibody sandwich method to determine the level of human metalloproteinase tissue inhibitory factor-1 (TIMP-1) in the specimen. Microporous plates were coated with purified human metalloproteinase tissue inhibitory factor-1 (TIMP-1) antibody to make solid-phase antibodies, and human metalloproteinase tissue inhibitory factor-1 (TIMP- 1), then combined with HRP-labeled metalloproteinase tissue inhibitory factor-1 (TIMP-1) antibody to form an antibody-antigen-enzyme-labeled antibody complex, and after thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with human tissue inhibitor of metalloproteinase-1 (TIMP-1) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human metalloproteinase tissue inhibitory factor-1 (TIMP-1) in the sample was calculated by a standard curve.