Fluorescent filter blocks are the core components of a fluorescence microscope with built-in excitation, emission, and transflective filters (also known as dichroic mirrors). When the fluorescent light source passes through the filter color block, the light of an excitation band is filtered, reflected by the transflective color filter, and concentrated by the objective lens, then enters the sample to be excited, and emits a specific wavelength of the fluorescence through the emission filter. , captured by the detector, forms a fluorescent image, and completes fluorescence detection. Simply composed of 3 color filters: (1) Excitation filter: Selecting a specific wavelength of light from a light source emitted from a mercury lamp, such as 340-380 band pass, means that light of a wavelength of 340-380 can pass through. (2) Spectroscopic dichroic mirror: The function is to reflect short-wavelength light (excitation light) and long-wavelength light (fluorescence). For example, 400nm splitting means that light reflection below 400nm is illuminated by the objective lens, and the excited fluorescence is transmitted through the dichroic mirror to the emission filter. (3) Emission filter: used to select the specific emission of the dye. For example, 415 long pass means that light transmission is greater than 415 nm, and light below 415 nm is completely blocked. The main function is to block the fluorescence or reflected light of other dyes. In fact, the microscope manufacturers do not produce filters, but only purchase the finished products of the color filter manufacturers for processing. The famous three major color filter manufacturers: (1) Chroma Chroma was established in May 1991. It specializes in the production of UV, visible and near-infrared filters, including single bandwidth, multiple bandwidths, long pass, short pass filters, as well as spectroscopes and dichroic mirrors. And laser filters, etc. They feature precise spectral control by precisely depositing thin layers of two or three materials onto glass or other transparent materials under vacuum. Currently, Chroma is the leading supplier of three of the top four manufacturers and another secondary supplier. It can be seen that Chroma is in the fluorescent microscope color filter industry! (2) Omega Founded in 1969, Omega has focused on providing color filters for fluorescence microscopy, and its interference filters are world-class products. Widely used in a wide range of wavelengths from UV to IR; from band filters for spectrometry, clinical diagnostics, and long/short channel filters for color separation for fluorescence emission, television and photography, to applications The dichroic beam splitter, laser filter, analytical filter, etc., which are optimally reflected on a specific region of the spectrum and optimally transmitted in another region on a fluorescence microscope, have numerous products and are widely used in industry, agriculture, and biological sciences. The field of scientific research and education plays an incomparable role. Compared with Chroma, Omega's price is lower, so the market share is higher. (3) Semrock Semrock has three main products: optical filters for fluorescent devices, Raman device filters and laser analysis filters. All Semrock optical filters are coated with a hard ion beam. This product is very stable, so the company offers a five-year warranty. The top-of-the-line BrightLine fluorescent filter greatly improves the performance of the microscope; the highly discriminating RazorEdgea filter is designed for the Raman system; the unique ultra-wideband MaxMirror is a laser dichroic mirror. Compared with the above two companies, Semrock's product features that all the color filters are hard-coated and expensive. Moderation Board,Foldable Flipchart,Art Easel Stand,Digital Flipchart Dongguan Aoxing Audio Visual Equipment CO.,Ltd , https://www.aoxing-alr.com