ã€Fundamental】 The free state of Coomassie Brilliant Blue G250 is reddish brown in acidic solution. It is blue when combined with protein. The protein content is proportional to the depth of color. After 595nm measurement, the standard curve of protein content and absorbance can be obtained. The protein concentration of the unknown sample. ã€equipment】 Visible spectrophotometer 2. Test tube ã€Reagents】 1.0.9% NaCl 2. Protein standard solution (500μg/ml): 50mg of calf serum albumin plus normal saline to 100ml 3. Coomassie Brilliant Blue G250 solution: Coomassie Brilliant Blue G250 100 mg plus 95% ethanol 50 ml, add 85% (W/V) H3PO4 100 ml plus distilled water to 1000 ml, place a brown bottle overnight, and filter with double-layer filter paper. ã€Steps】 (1) Drawing of standard protein curves 1. Take 7 small test tubes and operate according to the following table. 2. After mixing, let stand for 5 min, with No. 1 as blank and 595 nm colorimetric. 3. Results processing: The optical density of each tube is plotted on the ordinate. The amount of protein contained in each tube is plotted on the abscissa and a standard curve is prepared. (two) determination of protein content 1. Press the table below 2. After mixing and standing for 5 min, the blank tube was adjusted to zero, and the color ratio was 595 nm at room temperature, and the protein content in the sample was determined according to the standard curve. Shanghai Chuangsai Technology has excellent performance, interleukin cytokines, fetal bovine serum, electrophoresis equipment scientific instruments, raw material drug standards, chemical reagents, cell culture consumables, Shanghai Chuangsai, mass products special promotions, welcome to inquire!
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