Phosphatidylserine (PS) is normally located inside the cell membrane, but in the early stage of apoptosis, PS can be flipped from the inner side of the cell membrane to the surface of the cell membrane and exposed to the extracellular environment. Annexin-V is a Ca2-dependent phospholipid binding protein with a molecular weight of 35~36KD, which can specifically bind to PS with high affinity. Annexin-V was labeled with fluorescein (FITC, PE) or biotin, and labeled Annexin-V was used as a fluorescent probe to detect apoptosis by flow cytometry or fluorescence microscopy. Shanghai Chuangsai Technology provides BD antibody 550589, PE Goat Anti-Mouse Ig, product number: BD-550589-0.2 mg, inquiry. Propidine iodide (PI) is a nucleic acid dye that does not penetrate intact cell membranes. However, in advanced cells and dead cells of apoptosis, PI can pass through the cell membrane to redden the nucleus. Therefore, when Annexin-V is matched with PI, cells in early and late apoptotic cells and dead cells can be distinguished. Shanghai Chuangsai Technology provides propidium iodide, Propidium Iodide, product number: B11000383-10mg, price 172.2 yuan. method 1 Suspension of suspension cells: 1 Washing the normal cultured and induced apoptosis cells (0.5~1×106) twice with PBS; 2 Add 100ul Binding Buffer and FITC-labeled Annexin-V (20ug/ml) 10ul; 3 at room temperature in the dark for 30min, then add PI (50ug / ml) 5ul, after 5min in the dark, add 400ul Binding Buffer; 4 Immediately using FACScan for quantitative detection by flow cytometry (generally no more than 1 h), and a tube without Annexin V-FITC and PI as a negative control. Shanghai Chuangsai Technology provides fluorescein isothiocyanate, FITC, 3326-32-7, commodity number: B11000218-50mg, price 172.2 yuan. 2 Cell staining of adherent culture: The cells were first washed with 0.25% trypsin, washed, stained and analyzed for the same suspension cells. 3 Climbing cell staining: Ibid., finally observed with a fluorescence microscope and a confocal laser scanning microscope. Precautions 1. The entire operation should be as gentle as possible, do not force the cells. 2. Take care to avoid light during operation and test within one hour as soon as possible after the reaction is completed. CANSPEC quality provides excellent performance, its excellent quality to meet the various needs of your different experiments, spot, product details, scientific instruments, laboratory supplies, Shanghai Chuangsai, mass products special promotions, welcome to inquire! Furniture Custom,Customised Furniture,Customized Furniture,Customs Furniture BOSA FURNITURE CO.,LTD. , https://www.bosafurniture.com